Scientist/Senior Scientist, Characterization of Biologics by LC-MS Research Oxford Science Park

Enara Bio's purpose is to shine a light on unconventional T cell targets to develop cancer immunotherapies designed to provide lasting benefit for broad patient populations. Our proprietary EDAPT platform enables us to discover and validate novel and differentiated cancer-specific targets from the genomic dark matter, which we call Dark Antigens. We are advancing a pipeline of bispecific T-cell engagers against novel Dark Antigens and other underexploited targets for the treatment of patients with solid tumours.

Enara Bio was founded in 2016 and has been well funded to invest in the build out and growth of our R&D efforts and our fantastic team. The company is based in Oxford, UK.

Role Overview

Enara Bio has established a best-in-classpipeline for the identification of novel, previously undiscovered antigens for development of immunotherapies, using mass spectrometry (MS)-based proteomics and immunopeptidomics. We have recently broadened the application of MS to include the characterisation of biologics and are looking to increase internal capabilities in this area.

We are looking for a highly motivated senior scientist to join our team who has a passion for MS-based proteomics applied to the exciting field of immuno-oncology. The successful candidate will help in the generation of proteomics data to support the characterization of Enara Bio's novel biotherapeutics as well as supporting our ongoing antigen-discovery pipeline. This role will make a major contribution in supporting our goal of delivering impactful therapies that make a meaningful difference to patients suffering from cancer.

The successful candidate will work closely with key internal and external stakeholders including academic and non-academic partners in immunobiology, biochemistry, protein characterisation and LC/MS-based disciplines. Enara Bio actively promotes a multidisciplinary collaborative approach to working, therefore effective communication is essential to this role as you will be expected to effectively communicate with our collaborators about our scientific research objectives.

Key Responsibilities

• Provide technical leadership on various proteomics analytical workflows with experiencein different LCMS based proteomic methods (DDA, DIA, P(M)RM and intact mass spec)
• Drive the protein characterisation pipeline by applying various LC-MSbased techniques for our lead biotherapeutic products
• Support the existing immunopeptidomic and proteomic activities including the discovery and validation of targets by LC-MS
• Provide input for continuous development of MS-based proteomics and immunopeptidomic methodologies by working cross-functionally with Enara's protein characterisation and computational biology teams
• Provide technical expertise supporting the maintenance and troubleshooting of the LC-MS instrumentation
• Analyse, interpret and clearly communicate data to various stakeholders to support discovery and validation campaigns
• Contribute to a vibrant and innovative working environment where improvements and new ideas are adopted and freely shared among the teams

Minimum Qualifications and Capabilities

• Ph.D. (or equivalent experience) in a relevant scientific discipline such as MS-based proteomics
• In-depth technical expertise in LC-MS based proteomics and related sample preparation

Experience & Key skills:

• A track record of working independently and with the ability to design and execute experiments supporting the characterisation of biologics (Peptide fingerprint mapping, PTM analysis, disulfide bridge mapping and host cell proteomics (HCP) analysis etc)
• Proven ability to develop and maintain high resolution LC-MS instrumentation and use of proteomics software tools (such as PEAKS, DIANN, Skyline etc)
• Experience of working with Bispecifics and/or mAb's would be advantageous
• Experience with developing and troubleshooting complimentary protein characterisation techniques (IEX, HIC, SEC)
• Experience with sample preparation for proteomics analysis from biological samples including tissues and cell lines with knowledge of different enrichment strategies
• Effective communication, both written and verbal, with a good attention to detail
• Self-motivated and enthusiastic team player with experience of working in cross-functional teams and proven ability to work to specific deadlines

Desirable

• Experience of LC-MS based immunopeptidomics
• An understanding of the regulatory environment around biotherapeutics (ICH Q6B, ICH Q2 etc)
• Experience using R for data interrogation
• Cell culture experience

Further information:

The Enara Bio team is driven to make a meaningful difference for people affected by cancer. Our culture is defined by our behavioural ethos:

Empowerment: We promote autonomy across Enara rejecting conventional hierarchy. We engage each other through transparency, collaboration and trust. We each take action with a deep sense of personal and shared accountability.

Courage: We push boundaries in all that we do to deliver on our purpose. We make bold decisions, embrace risk and tackle challenges to advance our novel science. Courage is essential for everything we do.

Humility: We are proud of and excited by our novel science, but we acknowledge that we cannot know everything. Our curiosity drives us to continually learn from each other & the outside world irrespective of our role or title. We give and receive feedback with no room for ego.

Growth: We invest in our people, culture and community to foster belonging as a foundation for success. We embrace individual development to create deeper personal fulfilment and drive stronger collective impact.

We offer a competitive salary, commensurate with qualifications and experience, and a benefits package including pension and health insurance.

Applicants should be able to demonstrate proof of the right to work in United Kingdom.

Enara Bio Limited is an equal opportunities employer.